Effects of Rosiglitazone on Proliferation and Collagen I Synthesis of Heptic Stellate Cells

Objective To investigate the effects of rosiglitazone on the proliferation and collagen I synthesis of cultured hepatic stellate cells(HSC)in vitro.Methods HSC line,HSC-T6 was incubated with different concentraion of rosiglitazone(5,10,20 μmol/L).Cell proliferaeion was examined by MTT colormetric assay.Cell culture medium was used to detect collagen I level with ELISA method.Results Compared to control group,10,20 μmol/L rosiglitazone groups can inhibit HSC proliferation(P0.05).The collagen I level of 10,20 μmol/L rosiglitazone groups were 135.72±6.00 ng/ml,128.65±5.34 ng/ml respectively,which were significantly lower than control group(149.35±6.65 ng/ml,P0.05).Conclusion Rosiglitazone can inhibit HSC proliferation,decrease the collagen I synthesis.