Experimental study on the expression of connective tissue growth factor and protein kinase C in myocardium of Diabetic Rats

Objective: To evaluate the role of CTGF in the pathogenesis of diabetic cardiomyopathy and the relation of PKC,CTGF and interstitial fibrosis,and to provide the evidence of the treatment in DCM state.Methods:Sprague-Dawley(SD) rats were divided into 4 groups randomly:the normal control group(N4,N8) and the diabetic group(D4,D8).Diabetes was induced by Streptozotocin(STZ) intraperitoneal injection in SD rats of diabetic group.The protein expression of CTGF,PKC and FN were compared between the two groups of various duration by immunohistochemistry method.The RT-PCR was employed to detect the gene expression of CTGF.Results:Notable upregradulation of CTGF protein coincide with an apparent increase of PKC and FN expression in diabetic myocardium.These alterations were enhanced gradually along with longer diabetic duration;The expression of CTGF mRNA was enhanced gradually along with longer diabetic duration.Conclusions:In diabetic myocardium,PKC is enhanced gradually along with longer diabetic duration.It promotes the expression of FN by CTGF,and causes myocardium fibrosis.