Analysis on genetic diversity of rice varieties in Heilongjiang Province by using SSR

Genetic diversity of 54 rice varieties or lines of Heilongjiang Province,which were bred in recent years or were local cultivars in the past or introduced from Japan,were analyzed by using a set of 52 SSR molecular markers located on 12 chromosomes of rice.The results indicated that there was significant or highly significant genetic difference in 20 investigated phenotypic characters.The variance coefficient of setback was maximum,the rate of head milled rice and the breakdown took second place,the rate of brown rice and the pasting temperature were minimum.Total 45 of 52 SSR primers performed polymorphism,by which 160 alleles loci were detected with average 3.6.Shannon's index ranged from 0.04-0.702 with average 0.285.SSR primer polymorphic analysis showed high-polymorphic loci emerged mainly at chromosome 2,5,7 and 11 while low-polymorphic loci emerged at chromosome 6,8,10 and 12.The genetic similarity of paired varieties ranged from 0.667-0.963 with average 0.786,and the genetic similarity of 83.4% tested matetials ranged from 0.740-0.840,which had closer genetic relationship with each other.The results of UPGMA indicated that the 54 rice varieties could be clustered into three groups and the overwhelming varieyies were clusrered into the same group.