Intergration and Expression of Human Decay Accelerating Factor Gene in Transgenic Mice

To study the regulation of integration and expression for hDAF gene in transgenic mice,a DNA fragment contains hDAF gene from pSFFV DAF was isolated and then injected into the fertilized eggs of mice using pronucleus microinjection approach. When newborn mice are 4 weeks old, the genomic DNA was isolated from their tail. Dot blot and Southern blot hybridization were performed to identify the founder transgenic mice. Dot blot hybridization was used to analyse intergration of hDAF gene in the offspring from founder mice, and Northern blot hybridization was used to test expression of hDAF gene in transgenic mice.24 mice were born after microinjection.Four of them were ascertained as transgenic mice for hDAF gene, and the intergration frequency was 17%. There are respectively 70% and 75% of offspring mice from founder mice were integrated with hDAF gene. hDAF gene can be expressed at RNA level in kidney of 1 transgenic mouse. It shows hDAF gene integrated into genomic DNA of mice, and can be heredited and expressed in transgenic mice.