2013•Zhongguo yaowu yilaixing zazhiRequires access
A RAPID QUALITATIVE METHOD FOR URINE ANALYSIS WITH LC-MS/MS TO DISCRIMINATE HEROIN ABUSER
Zhang Daming
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Abstract
Objective:To establish a method of high-performance liquid chromatography-tandem mass spectrometry(LC-MS/MS)for discriminating heroin abusers rapidly.Methods: Urine samples of 162 heroin abusers and two experimental groups at different time points after they took the compound liquorice tablets and Codeine and Guaifenesin Syrup were determined by liquid chromatography-tandem mass spectrometry(LC-MS/MS),and performed descriptive statistics analysis.Results: Morphine,codeine,M3G and C6G and other opioids were detected in Urine samples from two experimental groups,and arrived peak concentration in 0.5-3.0 h.But the ratio of total morphine to total codeine(Mor/Cod)are different.The ratio of Mor/Cod in urine was between 1.61±s 0.67 and 3.07±s 1.85 after taking Compound Liquorice Tablets.However the value of Mor/Cod was between 0.09±s 0.07 and 0.24±s 0.12 after taking Codeine and Guaifenesin Syrup.The results of urine samples from heroin abusers revealed that 6-MAM was existed sometimes.A few samples contained heroin or acetyl-codeine prototype.There were nine samples whose ratios of Mor/Cod are below 1 among ninety urine samples,and the ratio of others was over 1.Conclusion: This method was feasible relatively and it is suitable to discriminate heroin abusers and people taking opioids.