[Determination of two bio-active compounds in Campylotropis hirtella (Franch.) Schindl. using reversed-phase high performance liquid chromatography].

A method of reversed-phase high performance liquid chromatography (RP-HPLC) using diode array detection (DAD) was developed for the quantitative determination of 3'-geranyl-5,7,4'-trihydroxyisoflavone (compound 1) and 8,9-dihydroxy-1-methoxy-[6',6'-dimethylpyrano(2',3': 2,3)]pterocarpene (compound 2) in Campylotropis hirtella. The separation and quantification were achieved using an Agilent Zorbax SB-C18 column (250 mm x 4.6 mm, 5 microm), and mobile phases of acetonitrile and 0. 1% formic acid with gradient elution at a flow rate of 1.0 mL/min and 30 degrees C. The calibration curves for compounds 1 and 2 were linear in the ranges of 4.4-13.2 microg and 0.428-1.284 microg, respectively. The recoveries were 99.65% and 99.11% with the relative standard deviations of 1.83% and 2.59% (n=5), respectively. This RP-HPLC-DAD method is rather simple, accurate and convenient. It can be used for the quantitative determination of the active flavonoids in Campylotropis hirtella (Franch.) Schindl.