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2007•Strait Pharmaceutical JournalRequires access

Study on dynamic variation of three flavonoids contents in folium eucommiae harvested from different seasons

Yang Zhong-lin

Open publisher page 2 citations

Abstract

OBJECTIVE To establish a HPLC method to determine the contents of rutin、quercetin and kaemperal in folium eucommiae, and to ensure the best harvest season. METHODS Scienhome C18(150×4. 6mm,5μm)column with temperature at 25℃was used. The mobile phase was methanol and 0. 5%H3PO4,at a flow rate of 1. 0ml·min-1,and the detection wavelenth was 270nm. RESULTS The average recovery and RSD of the three flavonoids contents respectively was:rutin 98. 44%,1. 20%;quercetin 100. 81% ,0. 69%;kaemperal 98. 39%, 1. 00%. The content of flavonoids was highest in May. CONCLUSION Our method of HPLC is effective and the most suitable harvest season of folium eucommiae is May.

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What this paper is about

OBJECTIVE To establish a HPLC method to determine the contents of rutin、quercetin and kaemperal in folium eucommiae, and to ensure the best harvest season. METHODS Scienhome C18(150×4. 6mm,5μm)column with temperature at 25℃was used. The mobile phase was methanol and 0. 5%H3PO4,at a flow rate of 1. 0ml·min-1,and the detection wavelenth was 270nm. RESULTS The average recovery and RSD of the three flavonoids contents respectively was:rutin 98. 44%,1. 20%;quercetin 100. 81% ,0. 69%;kaemperal 98. 39%, 1. 00%. The content of flavonoids was highest in May. CONCLUSION Our method of HPLC is effective and the most suitable harvest season of folium eucommiae is May.

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Available abstract

OBJECTIVE To establish a HPLC method to determine the contents of rutin、quercetin and kaemperal in folium eucommiae, and to ensure the best harvest season. METHODS Scienhome C18(150×4. 6mm,5μm)column with temperature at 25℃was used. The mobile phase was methanol and 0. 5%H3PO4,at a flow rate of 1. 0ml·min-1,and the detection wavelenth was 270nm. RESULTS The average recovery and RSD of the three flavonoids contents respectively was:rutin 98. 44%,1. 20%;quercetin 100. 81% ,0. 69%;kaemperal 98. 39%, 1. 00%. The content of flavonoids was highest in May. CONCLUSION Our method of HPLC is effective and the most suitable harvest season of folium eucommiae is May.

Key concepts: Folium of Descartes, Rutin, Quercetin, Chemistry, High-performance liquid chromatography, Flavonoid, Chromatography, Food science

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