Baseline sensitivity of Colletotrichum gloeosporioides and C. capsici from capsium to azoxystrobin
Mingguo Zhou
Abstract
Mingguo Zhou
Abstract
single spore isolates causing capsium anthracnose collected from Jiangsu and Hainan provinces were identified into C. gloeosporioides and C.capsici based on the morphological characteristics. Of these isolates, 64.4% was C. gloeosporioides . Sensitivity of the C.gloeosporioides and C. capsici isolates to azo- xystrobin was determined on AEA and WA media suitable for conidium production and for spore germination test,respectively. The mean EC 50 value of the azoxystrobilurin inhibiting spore germination of the 45 isolates of both species was 0.047 μg/mL with a standard error of 0.040 and a range of 0.009 to 0.091 μg/mL. The mean EC 50 values of 29 isolates of C. gloeosporioides and 16 isolates of C. capsici isolates were (0.051±0.047) μg/mL and (0.041±0.024) μg/mL,respectively. There was coordinate synergism between azoxystrobin and salicylhydroxamic acid(SHAM) as an inhibitor of alternative oxidase against spore germination of C.gloeosporioides and C.capsici . However,azoxystrobin and SHAM inhibited mycelial growth solely and azoxystrobin was much less effective against mycelial growth.
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single spore isolates causing capsium anthracnose collected from Jiangsu and Hainan provinces were identified into C. gloeosporioides and C.capsici based on the morphological characteristics. Of these isolates, 64.4% was C. gloeosporioides . Sensitivity of the C.gloeosporioides and C. capsici isolates to azo- xystrobin was determined on AEA and WA media suitable for conidium production and for spore germination test,respectively. The mean EC 50 value of the azoxystrobilurin inhibiting spore germination of the 45 isolates of both species was 0.047 μg/mL with a standard error of 0.040 and a range of 0.009 to 0.091 μg/mL. The mean EC 50 values of 29 isolates of C. gloeosporioides and 16 isolates of C. capsici isolates were (0.051±0.047) μg/mL and (0.041±0.024) μg/mL,respectively. There was coordinate synergism between azoxystrobin and salicylhydroxamic acid(SHAM) as an inhibitor of alternative oxidase against spore germination of C.gloeosporioides and C.capsici . However,azoxystrobin and SHAM inhibited mycelial growth solely and azoxystrobin was much less effective against mycelial growth.
Key concepts: Azoxystrobin, Salicylhydroxamic acid, Mycelium, Spore germination, Spore, Colletotrichum capsici, Germination, Horticulture