Construction of Recombinant Adenovirus Expressing Influenza Virus Haemagglutinin

The HA gene of influenza virus A1/PR8/34 was amplified by RT-PCR and cloned into the shuttle plasmid pAd5C. The constructed plasmid and the largest fragment of adenovirus type 5 genome DNA digested by EcoR I were cotransfected into 293 cells. Recombinant virus was selected by PCR. The expression of HA gene in the cells infected with recombinant virus was identified by SDS-PAGE and Westen blot. Mice immunized with the recombinant virus by nasal route can produce HA specific antibody both in serum and on mucosal surface. The Ad5 vector with E3 region deletion can be used as a vector to construct the recombinant influenza virus vaccine.