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2012•Unpublished venueRequires access

The expression and significance of HIF-1α and VEGF in hypoxic neurons

Juan Du

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Abstract

Objective To investigate the expression pattern and significance of hypoxia-inducible factors 1α(HIF-1α)and vascular endothelial growth factor(VEGF)in primary neonatal rat cortical neurons at different periods of hypoxia.Methods Primary cultured neurons were exposed to hypoxia for 0,2,4,8 hours.The expressions of HIF-1α and VEGF were detected by Western blot and real-time PCR.Results The HIF-1α was rarely detected and the expression of VEGF was low in normoxia.The mRNA expressions of HIF-1α and VEGF were significantly increased after 4 hours of hypoxia.The levels of HIF-1α and VEGF peaked at 4 hours then declined to near basal level at 8 hours.Conclusions The levels of HIF-1α and VEGF are increased in hypoxia.The expressions increase initially and then decline as the hypoxic time extends.

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Objective To investigate the expression pattern and significance of hypoxia-inducible factors 1α(HIF-1α)and vascular endothelial growth factor(VEGF)in primary neonatal rat cortical neurons at different periods of hypoxia.Methods Primary cultured neurons were exposed to hypoxia for 0,2,4,8 hours.The expressions of HIF-1α and VEGF were detected by Western blot and real-time PCR.Results The HIF-1α was rarely detected and the expression of VEGF was low in normoxia.The mRNA expressions of HIF-1α and VEGF were significantly increased after 4 hours of hypoxia.The levels of HIF-1α and VEGF peaked at 4 hours then declined to near basal level at 8 hours.Conclusions The levels of HIF-1α and VEGF are increased in hypoxia.The expressions increase initially and then decline as the hypoxic time extends.

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Available abstract

Objective To investigate the expression pattern and significance of hypoxia-inducible factors 1α(HIF-1α)and vascular endothelial growth factor(VEGF)in primary neonatal rat cortical neurons at different periods of hypoxia.Methods Primary cultured neurons were exposed to hypoxia for 0,2,4,8 hours.The expressions of HIF-1α and VEGF were detected by Western blot and real-time PCR.Results The HIF-1α was rarely detected and the expression of VEGF was low in normoxia.The mRNA expressions of HIF-1α and VEGF were significantly increased after 4 hours of hypoxia.The levels of HIF-1α and VEGF peaked at 4 hours then declined to near basal level at 8 hours.Conclusions The levels of HIF-1α and VEGF are increased in hypoxia.The expressions increase initially and then decline as the hypoxic time extends.

Key concepts: Hypoxia (environmental), Vascular endothelial growth factor, VEGF receptors, Western blot, Endocrinology, Messenger RNA, Internal medicine, Basal (medicine)

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