Effect of bone marrow stromal cell line transduced IL-3 on acceleration of the hematopoietic function in v itro

ObjectiveTo observe whether bone marrow stromal cell line QXMSC1 engineered to secrete IL-3 (QXMS1 IL-3) can accelerate the hematopoietic function in vitro.MethodsQXMAC1 IL-3 cell was constructed with transduiced mIL-3 using retrovirus vector and the highest secreting cells was used in the following expriments.Short-term culture was used to observe CFU-GM,CFU-E?CFU-GEMM in adding QXMSC1 IL-3 supernatant.Longterm culture-initiating cells (LTC-IC) was measured and then separated from the stromal layer by micro-porous membrane to observe hematopoietic progenitors in the absence of stromal cells QXMSC1 IL-3.ResultsQXMSC1 IL-3 significantly increased the number of CFU-GM,CFU-E and CFU-GEMM in short-term culture as compared with QXMSC1 and also improved the production of granulocyte-macrophage progenitors in long term culture.Though the absence of direct contact between hematopoietic function and stromal QXMSC1 IL-3,the number of nucleated cells and CFU-GM decreased,still significantly increased than that of QXMSC1 and QXMSC1 plxsn.ConclusionThese data demonstrated that the bone marrow stromal cell line QXMSC transduced with IL-3 (QXMSC1 IL-3) can improved the hematopoietic function in vitro and may be used in cell therapy and gene therapy in animal models.