PCR-RFLP Analysis of 3'-UTR of myogenin Gene in Pigs

The genetic variations of 3'-UTR of myogenin gene were detected with PCR-RFLP-MspⅠ in 630 pigs including Large Yorkshire, Landrace, Duroc, Ningxiang pig, Shaziling pig, Daweizi pig and Taoyuan black pig. The results showed that the genotypes of AA, AB, BB existed in the exotic breeds and the genotype of BB was dominant, and the frequency of allele B was 0.74. Three genotypes also existed in the Chinese native breeds, and the genotype of AA was dominant, and the frequency of allele A was 0.95. The frequencies of allele A and B were remarkably different between the exotic breeds and Chinese native breeds. The χ2 value of genotypes indicated that every exotic pig breed had highly significant differences with four native pig breeds'. Taoyuan pig had highly significant differences with Ningxiang pig, Shaziling pig and Daweizi pig. Daweizi pig had significant differences with Ningxiang pig and Shaziling pig. Large Yorkshire had highly significant differences with Landrace pig.