Preliminary studies on DNA vaccine of cDNA encoding the conservative region of gynecophoral canal protein of Schistosoma japonicum(Chinese strain)

Aim In order to observe the protective efficence induced by vaccinating animals with the DNA vaccine of Sex specific expression gene of Schistosoma Methods A 868 bp cDNA fragment amplified by RT PCR from adult Schistosoma japonicum(Chinese strain) mRNA was cloned into the eukaryotic expression vector pcDNA3 and the recombinant eukaryotic expression vector pcDNA3 SjGCP1 was directly injected into Kuming mice intramuscularly 3 times with the interval of 5 weeks Both the vaccinated and control group of mice were challenged with 40 cercariae of Sj 5 weeks after last injection and perfused 7 weeks post challenge Results The worm and egg counting reduction got from vaccinated mice was 32 4% and 46 9% respectively Conclusion The result indicates that pcDNA3 SjGCP1 DNA vaccine induces the significant protection in animal against Schistosoma japonicum infection