Molecular cloning of DNA sequences encoding Krt71 gene in SD rats

This study was designed to clone and analyze the DNA and cDNA encoding Krt71 gene from SD rats.The PCR method was developed to clone the Krt71 DNA and cDNA.A full-length DNA sequences of SD rat was 8 206 bp,which has been accepted by GenBank(Accession Number:KF311105),and a full-length cDNA sequences of SD rat were 1 575 bp,which has been accepted by GenBank(Accession Number:KF303589).Krt71 protein encoded by this gene was composed of 524 amino acid residues.The identities of cDNA sequences of Krt71 gene were99.9%,95.2%,88.5%,87.5%,88.0%,88.1% and 88.1% by homologous comparison among SD rat and other species,and in amino acid sequences were 100.0%,98.9%,92.5%,91.5%,91.3%,91.5% and 92.1%,respectively.The result suggested a high degree of conservation of the Krt71 gene among different mammalian taxons.The results derived from information searching by Blast program revealed there were 6SNP sites in the sequences of Krt71 gene DNA between SD rats and that collected in GenBank.These SNP sites were located in the intron sequences,and they did not alter the related amino acids encoded.The results provided news content for SNPs in Krt71 gene.