Cloning and Genetic Analysis on Interleukin-2 Gene of Huainan Pig

One pair of primers was designed based on the pig IL-2 gene sequences published in GenBank and used to amplify the interleukin-2 cDNA of Huainan Pig by RT-PCR from total RNA extracted from spleen lymphocytes,which were cultivated and stimulated with 10 μg/mL ConA in vitro for 27 h.Then the amplified cDNA was cloned into pGEM-T Easy vector and sequenced.The result showed that the full length of Huainan pig IL-2 was 516 bp with an ORF of 465 bp,encoding a peptide of 154 amino acids which molecular weight is 17.4 kDa,pI 5.27,hydrophobic amino acid 38.3%,hydrophilic amino acid 42.0%,basic amino acid 11% and acidic amino acid 23%.The sequence was the same as the corresponding genes published in Genebank.The base similarity of pig IL-2 with those of cat,cattle,chicken,dog,duck,goat,horse,human and house mouse was 83.7%,82.6%,28.2%,80.6%,29.6%,83.4%,81.3%,82.0% and 61.5%,respectively.