Intervenient effect of 764-3 on TF expression induced by tumor necrosis factor-α in endothelial cells

To investigate the intervenient effect of 764-3 on TFexpression and calcium ion([Ca 2+]i) induced by TNF-α in human vascular endothelial cells. We constructed two luciferase reporter gene containing different upstream sequences of TF gene. The two luciferase report genes, together with the intracontrol plasmid pSV-β-gal were respectively cotransfected into cultured human vascular endothelial cells. The relative luciferase activities were detected in ECV304 treated by TNF-α or/and 764-3. [Ca 2+] i in single HUVEC was observed with Laser-scanning confocal Microscope. TNF-α significantly increased the luciferase expression in the p-244/+121bp Luc transfected endothelial cells, the effect of TNF-α could be inhibited by 764-3. The luciferase expression in the p-111/+121bp Luc transfected endothelial cells was lower than that in the p-244/+121bp Luc transfected endothelial cells. TNF-α increase [Ca 2+] i in single HUVEC, but it was inhibted by 764-3. TF gene expression induced by TNF-α was inhibited by 764-3. [Ca 2+] iwas involved in it.