Study on extraction of peanut protein from heat treated peanut meal
Li Yan
Abstract
Li Yan
Abstract
Alkaline-extraction and acid-precipitation combined with pretreatment were employed to isolate peanut protein from heat treated peanut meal.The results of quadratic regression rotatable orthogonal design showed the optimum conditions of isolation were as follows:the alkaline-extraction was at 60℃,pH9.5 for 120min with the ratio of solid to water at 1:8(m/v).Under these optimal conditions,the extraction rate of peanut protein was 64.2%.High-speed homogenization or ultrasonic pretreatment could both raise the extraction rate significantly,especially through ultrasonic treatment,it obviously increased by 12.3% compared with those without pretreatment.However,pretreated by cellulase had no effect on it.
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Alkaline-extraction and acid-precipitation combined with pretreatment were employed to isolate peanut protein from heat treated peanut meal.The results of quadratic regression rotatable orthogonal design showed the optimum conditions of isolation were as follows:the alkaline-extraction was at 60℃,pH9.5 for 120min with the ratio of solid to water at 1:8(m/v).Under these optimal conditions,the extraction rate of peanut protein was 64.2%.High-speed homogenization or ultrasonic pretreatment could both raise the extraction rate significantly,especially through ultrasonic treatment,it obviously increased by 12.3% compared with those without pretreatment.However,pretreated by cellulase had no effect on it.
Key concepts: Extraction (chemistry), Chemistry, Protein purification, Homogenization (climate), Cellulase, Meal, Food science, Chromatography