Induce apoptosis effect of Ginseng Rh2 on HepG2 cells

Objective To investigate the effects of genoside Rh2 on inducing hepatocellular carcinoma cell hepG2 apoptosis in vitro.Methods MFC cells were divided into four gourps:cont rol group and G-Rh2(5,10,20 mg/L) groups.The cell viability was determined by MTT;the cell cycle was detected by flow cytomet ry;the gene expression of survivin was observed by RT-PCR.Results Compared with cont rol group,the cell viabilities decreased gradually with the increasing of dose and time in G-Rh2(5,10,20 mg/L) groups at different time(24,48,72 h)(P0.05),the ratio of FCM G0/G1 cells increased and S cells decreased(P0.05),at the same time,the expression level of surviving decreased in a dose2dependent manner(P0.05).Conclusion G-Rh2 can decease cell viability by inducing apoptosis and arresting cell cycle,and the process may associate with survivin decrease.