EFFECT OF LOW FAT DIET ON EXPRESSTION OF PTOTEIN KINASE B IN ADIPOSE TISSUE OF RATS WITH INSULIN RESISTANCE

Objective: To study the effect of low fat diet on expression of protein kinase B (PKB) in adipose tissue of rats with insulin resistance. Method: Thirty male Wistar rats were randomly divided into control group ( C, n=10),given low fat diet; model group (M, n=20), given high fat diet. After 4 w, group M was randomly divided into 2 subgroups accepting diet intervention of 6 w.(1)High fat group (HF) was continually given high fat diet;(2)Low fat group (LF) accepted low fat diet. The expression of PKB stimulated by insulin in adipose tissue was determined with Western blotting at the end of the experiment. Results: (1) Group M showed hyperglycemia, hyperinsulinism, hypertriglyceride, hypercholesterolemia and elevated HOMA insulin resistance index (HOMA-IR) while the insulin sensitivity index (ISI) was obviously reduced compared to group C, indicating that the insulin resistant model was induced ; (2) Low fat diet treatment decreased FBG,TG,TC,which were accompanied by lower HOMA-IR and elevated ISI; (3) Because of long term high fat diet, expression of PKB in adipose tissue of group HF decreased by 23.5% (P0.01)compared with group C. There was an increase of 16.1%(P0.01)in PKB expression of group LF compared to group HF. Conclusion: Low fat diet can significantly elevate expression of PKB and ameliorate the state of insulin resistance .