Studes on the alkaline protease from Bacillus licheniformis I. the screening of high yield alkaline protease strain and the conditions for enzyme production

A high yeild alkaline protease strain was isoated from samples . The strain was identified as Bacillus licheniformis , called B. L JF-ld. The optimum conditions for the protease production consisted of 7. 5% maltose , 3% yeastextract,0. 5% NaCl,0. 53% K2HPO4· 3H2O,0. 03% NaH2PO· 2H2O, 0. 056 % Na2CO3. 1 × 10-4 mol/L MnSO4,pH8. 7. After fermentation in 2L of fermentor at 37℃, with an aeration rate of(1: 0. 5) ~ (1: 1 ) (v/v) for 40 hours, theenzyme activity was about 7180U/ml of fermentation liquor.