AFLP analysis on genetic diversity of four different generations of Litopenaeus vannamei.

The AFLP technique was used to analyze the genetic diversity of 4 different generation groups of Litopenaeus vannamei,which were primary parent,F1 generation,F2 generation and Fn generation.10 pair AFLP selective primer scanned 20 samples from each generation and 483 loci were amplified,348 loci were polymorphic loci.The proportions of polymorphic loci among the 4 generation samples were 61.90% ,49.28% ,50.52% and 46.38% respectively.The genetic diversities of the 4 different generations quantified by Shannon index were 0.313 4,0.262 1,0.259 4 and 0.226 2 respectively.The result shows that the genetic distance between primary parent and F1 generation sample is closer than that between F2 and Fn generation samples.The research of genetic diversity among 4 different generations could provide theoretical support for future study on hybrid selective breeding.