Investigation of reference intervals of serum alanine aminotransferase and aspartate aminotransferase within healthy adult individuals in Kunming

Objective To establish the biological reference intervals of serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) within healthy adult individuals in Kunming.Methods According to the indirect sampling technique in Clinical and Laboratory Standards Institute(CLSI) C28-A2,ALT and AST of 9 890 healthy individuals were determined by Olympus AU5421 automatic biochemical analyzer from May 2010 to March 2011.The test results were statistically analyzed by Stata 11.Results The reference intervals(≤P95) were showed as follows: ALT ≤51 U/L(male) and ≤34 U/L(female),AST ≤40 U/L(male),≤35 U/L(female).In male and female group,the levels of ALT and AST were increased generally with ages.In male group,the highest level of ALT was showed in age 30-40. Conclusion The reference intervals of ALT and AST in Kunming are significantly different from those showed in reference books or from other districts.So it is necessary to establish the biological reference intervals of ALT and AST for each laboratory.