2004•Acta Entomologica SinicaRequires access

A new silver staining method for proteins of housefly in sodium dodecyl sulfate-polyacrylamide gel electrophoresis

An Chun

Abstract

A new Na 2S 2O 4 silver staining method is described for detecting the proteins of housefly separated by SDS-PAGE. In this staining method, besides the regents and steps are different from the classical silver staining method, fixation and ethanol washing are not necessary. Only twenty minutes were needed to complete the whole staining process. This method has a low demand for regents and manipulation, but can still reach the sensitivity level of nanogram.

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Key concepts: Staining, Housefly, Silver stain, Polyacrylamide gel electrophoresis, Chemistry, Sodium dodecyl sulfate, Chromatography, Fixation (population genetics)