Effects of titanium alloy particle on human osteoblasts in vitro

Objective To study the effects of particulates titanium alloy on the proliferation,viability of bone-forming of human osteoblasts in vitro.Methods Human bone marrow stormal cells(hMSCs)were obtained and induced to differentiate and proliferate into osteoblasts.The 3rd to 5th generations of human osteoblasts were cultivated with different concentrations of particulates titanium alloy(1.0,0.1 and 0.01 mg/ml).Cell growth and proliferation were detected by MTT method.Alkaline phosphatase(ALP)activity and collagen-I synthesis were measured by kit.Results The concentrations of 1.0 and 0.1 mg/ml titanium particle reduced cells proliferation(P0.05),whereas 0.01 mg/ml did not statistically change cells proliferation(P0.05).The three different concentrations of particle had effects on ALP activity and collagen-I synthesis significantly(P0.05)with some degree of dose-and time-effect relationship.Conclusion Titanium alloy particles reduce cell proliferation and suppress cell osteogenesis,which may be one of the mechamisms for the periprosthetic osteolysis.