2001•PubMedRequires access
[Comparison of denaturing high performance liquid chromatography with direct sequencing in the detection of single nucleotide polymorphism].
Jiaqi Shi, Shi‐Yi Yang, Zhi Liang Jiang, Hongmei Jiang, T Chen, Z Chen, Weiyuan Huang
Open publisher page 2 citations
Abstract
OBJECTIVE: Investigate the sensitivity and accuracy of the denaturing high performance liquid chromatography(DHPLC) technique for the detection of single nucleotide polymorphism(SNP). METHODS: Forty-one samples were detected by both DHPLC and direct sequencing. RESULTS: The comparison demonstrated that DHPLC detected all heterozygous sequences found by direct sequencing. No false-positive signals were seen in the cases of homozygous sequences. Furthermore, no false-negative results were ever obtained with heterozygous mutations or polymorphisms, or both. CONCLUSION: DHPLC is a potent method for SNP identification especially SNP typing in large scale screening.