Study on human platelet antigen-1～-17bw genotyping by PCR sequencing based typing methods

Objective:To establish the human platelet antigen(HPA)system genotyping methods by direct polymerase chain reaction sequencing based typing(PCR-SBT).Method:Genome of HPA-1to HPA-17bw were amplified by PCR.Amplification products were sequenced directly.Result:No discrepancies were observed between routine polymerase chain reaction with sequence-specific primers(PCR-SSP)and developed SBT methods by us in the results of genotyping for a panel of HPA-well typed platelet donors that included all common HPA alleles and the rare HPA-1b,-2band-6bw homozygous.Conclusion:The PCR-SBT protocol for simultaneous genotyping of HPA-1to HPA-17bw was successfully established,which was an accurate and reliable method for characterizing HPA polymorphisms,as well as applied to platelet immunology and anthropogenetics study.