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2006•Chinese Journal of Food HygieneRequires access

Quantitative Determination of Biotin in Health Foods by HPLC

Xinzhi Zhang

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Abstract

A HPLC method was established for the determination of biotin in health foods. The HPLC used Hypersil ODS column,a mixture of CH3CN+0.05 mol/L KH2PO4(15+85,pH=3.5) as mobile phase and UV detection at 200 nm.The recovery was 86.8%～106.0%,The RSD was 8.4%,the detection limit was 0.5 mg/kg. The method is simple, accurate and reproducible.

About this research paper

What this paper is about

A HPLC method was established for the determination of biotin in health foods. The HPLC used Hypersil ODS column,a mixture of CH3CN+0.05 mol/L KH2PO4(15+85,pH=3.5) as mobile phase and UV detection at 200 nm.The recovery was 86.8%～106.0%,The RSD was 8.4%,the detection limit was 0.5 mg/kg. The method is simple, accurate and reproducible.

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Available abstract

A HPLC method was established for the determination of biotin in health foods. The HPLC used Hypersil ODS column,a mixture of CH3CN+0.05 mol/L KH2PO4(15+85,pH=3.5) as mobile phase and UV detection at 200 nm.The recovery was 86.8%～106.0%,The RSD was 8.4%,the detection limit was 0.5 mg/kg. The method is simple, accurate and reproducible.

Key concepts: Chromatography, High-performance liquid chromatography, Biotin, Detection limit, Chemistry, Health food, Biochemistry, Food science

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