The effection of lipo-PGE1 on LPS-induced acute lung injury in animals

Aim To ivestigate the role of Lipo-prostaglandin E1(lipo-PGE1) in regulation of lipopolysaccharide(LPS)-induced lung injury in animals.Methods Thirty-two SD rats were averagely and randomly divided into saline control group(n=8 each),LPS(n=12 each) and LPS+Lipo-PGE1 group(n=12 each).LPS model were intravenously injected with LPS(5mg/kg).The rats in PGE1 group were intravenously injected with PGE1(10μg/kg) ten minutes after LPS were injected into them.The concentrations of lactate and the numbers of white blood cells(WBC) were also analyzed;The pathological changes in lung tissues also in bronchoalveolar lavage fluids(BALF) were detected;The arterial blood gas and venous blood gas ere observed.Results In comparison with those in control group,the white blood cells(799.5±217.32 vs 219.17±102.17,P0.01) and total proteins(0.71±0.083 vs 0.2±0.059,P0.01) in bronchoalveolar lavage fluids(BALF) increased obviously.Compared with the LPS groups,the white blood cells(292.73±42.9 vs 799.5±217.32,P0.01) and total proteins(0.543±0.064 vs 0.71±0.083,P0.01);In comparison with those in control group,the blood lactate concentrations and blood gas were obviously changed.The values of A-VpH(0.10±0.027 vs 0.0720±0.043,P0.05) and A-VpCO2(26.41±10.49 vs 22.34±9.72,P0.05) and lactate concentration(4.36±2.78 vs 2.81±0.79,P0.05) were increased obviously.Compared with the LPS groups,The values of A-VpH(0.078±0.031 vs 0.10±0.027,P0.05) and A-VpCO2(21.92±5.84 vs 26.41±10.49,P0.05) and lactate concentration(3.86±1.39 vs,4.36±2.78,P0.05) were significantly decreased in the PGE1 groups;In LPS group,the progressively pathological damages in the lung tissues were clearly observed.Conclusion PGE1 plays an essential role in regulation of LPS-induced acute lung injury.The WBC counts and TP concentration in BALF were reduced and the pathological changes in lung tissues relieved.