PRELIMINARY RESEARCH ON BASELINE DATA OF DRUG RESISTANT MUTATIONS AMONG TREATMENTS OF NAIVE HIV INFECTED PERSONS IN SHENZHEN BY GENOTYPING METHOD

[Objective]To construct a complete and reliable HIV-1 genotyping method which was suited to the HIV positive population in Shenzhen that can be used to analyze the drug resistant mutations and prevalence of HIV-1 strains among them.[Methods]HIV-1 viral RNA samples were collected from plasma samples of HIV-1 infected persons without HAART and were amplified by nested PCR using specific primers;then the amplified fragments were sequenced and analyzed.[Results]The 1300bp fragments of RT-PCR products were obtained from the samples. Among 21 RT genes obtained from sequencing,there were 8 NRTI resistance mutations(38.5%)and 5 NNRTI resistance mutations(23.81%). In addition,there were 1 PI major resistance mutations(8.33%)and 5 PI minor resistance mutations(41.67%)from 12 PR genes results.[Conclusion]HIV-1 genotyping is an effective tool for the surveillance of HIV-1 drug resistant strains and the testing results confirm that there are HIV-1 drug resistant strains among HIV-1 infected persons without antiretroviral therapy in Shenzhen.