Yeast cell bioassay development based on environmental estrogen regulating β-galacosidase

To establish the bioassay system of environmental estrogen,the reporter plasmid of pMP206/ERE-LacZ was constructed by inserting two consensus ERE-sequences into the upstream of pMP206.The reporter vector was transformed into the human estrogen receptor recombinant yeast cell. In this way,the reporter gene,LacZ,was under regulation of estrogen.This yeast cell could be used to screen environmental estrogen.Results showed that DNA sequences revealed the reporter vector of pMP206/ERE-LacZ with the correct DNA fragment.The DNA fragments of estrogen gene and the pMP206/ERE-LacZ reporter plasmid were found with PCR method in the transformant.This exhibited that estrogen receptor and LacZ reporter gene had been recombined into the yeast cell.The yeast cell for environmental estrogen bioassay based on LacZ reporter gene was successfully recombined.