Effects-of Probucol on smooth muscle cell proliferation induced by H_2O_2

Objective: To investigate the inhibitory effects of Probucol on smooth muscle cells (SMCs) proliferation. Methods: SMCs treated with H_2O_2 and new born calf serum(NCS) served as proliferative cellular model in vitro. Inhibition effects of Probucol on the SMCs proliferation was detected by cell counting and incorporation of ~3H-TdR. Results: A does- dependent inhibition effects on the proliferation of SMCs induced by 10% NCS was observed when treated with 10 μmol/L and 100 μmol/L Probucol for 96 h (PO. 05, P0. 01 ). Does-dependent inhibition effects on proliferation of SMCs evoked by H_2O2_ was found when treated with 1 μmol/L, 10 μmol/L and 100 μmol/L Probucol for 72, 96 h (P0. 05, PO. 01 ). Con- clusion: Probucol inhibits SMCs proliferation stimulated by NCS or H_2O_2 Probucol may have potential clinical implication in the prevention and treatment of atherosclerosis and restenosis after percutaneous transluminal coronary angioplasty.