Optimization of Agrobacterium-Mediated Transformation System of Grape

In this paper,using the leaves of grape cultivar ‘Merlot'as transformation material,many influence factors including the concentration of carbenicillin, the concentration of Acetosyringone( AS), the concentration of Agrobacterium,the concentration of kanamycin were studied to screen the optimal system. The results show that,the300mg·L- 1carbenicillin could effectively remove the Agrobacterium and with rational differentiation of embryogenic callus,the optimal acetosyringone( AS) concentration was 50μm·L- 1,the concentration of Agrobacterium at 0. 5 ~0. 7 of OD600 had the highest transformation rate,and the optimal kanamycin concentration was 100mg·L- 1. By using the optimal agrobacterium-mediated transformation system,the regeneration plants were detected by PCR,and the results indicated that the exogenous gene was transformed into the grape genome. In this study,the factors affecting to Agrobacteriummediated transfermation of grape were optimized,and the transgenic plants were obtained.