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2006•Journal of Zhejiang University of TechnologyRequires access

Determination of 17α-hydroxy-16β-methyl-pregna-1, 4,9,(11)-triene-3,20-dione by dual wavelength spectrophotometry

Bingjun Yu

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Abstract

A rapid and simple method for quantitative determination of 17α-hydroxy-16β-methyl-pregna-1,4,9,(11)-triene-3,20-dione(HMPTD) was investigated for mixed samples of HMPDD and HMPTD with an absorbance-ratio method of dual wavelength spectrophotometry.It was found that the mass fraction HMPTD related linearly to the absorbance ratio A_(267)/A_(253).They can be correlated by using the following linear equation Y=3.05X-0.739 with a coefficient of determination of 0.9995.The method of dual wavelength spectrophotometry was used to analyze the samples obtained by microbial transformation in comparison with the HPLC method.The relative error was below 2%.This means that the proposed method was suitable for the measurement of the HMPDD conversion ratio for microbial dehydrogenation.

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What this paper is about

A rapid and simple method for quantitative determination of 17α-hydroxy-16β-methyl-pregna-1,4,9,(11)-triene-3,20-dione(HMPTD) was investigated for mixed samples of HMPDD and HMPTD with an absorbance-ratio method of dual wavelength spectrophotometry.It was found that the mass fraction HMPTD related linearly to the absorbance ratio A_(267)/A_(253).They can be correlated by using the following linear equation Y=3.05X-0.739 with a coefficient of determination of 0.9995.The method of dual wavelength spectrophotometry was used to analyze the samples obtained by microbial transformation in comparison with the HPLC method.The relative error was below 2%.This means that the proposed method was suitable for the measurement of the HMPDD conversion ratio for microbial dehydrogenation.

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Available abstract

A rapid and simple method for quantitative determination of 17α-hydroxy-16β-methyl-pregna-1,4,9,(11)-triene-3,20-dione(HMPTD) was investigated for mixed samples of HMPDD and HMPTD with an absorbance-ratio method of dual wavelength spectrophotometry.It was found that the mass fraction HMPTD related linearly to the absorbance ratio A_(267)/A_(253).They can be correlated by using the following linear equation Y=3.05X-0.739 with a coefficient of determination of 0.9995.The method of dual wavelength spectrophotometry was used to analyze the samples obtained by microbial transformation in comparison with the HPLC method.The relative error was below 2%.This means that the proposed method was suitable for the measurement of the HMPDD conversion ratio for microbial dehydrogenation.

Key concepts: Spectrophotometry, Absorbance, Chemistry, Chromatography, Analytical Chemistry (journal)

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Determination of 17α-hydroxy-16β-methyl-pregna-1, 4,9,(11)-triene-3,20-dione by dual wavelength spectrophotometry — Research Paper | ScholarLens