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Effects of miR-130a on chemosensitivity of human breast cancer cells to adriamycin by targeting PTEN

Jinhai Tang

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Abstract

OBJECTIVE:To investigate the effect of miR-130a on expression of PTEN,and their association with the chemosensitivity of MCF-7 breast cancer cells to adriamycin.METHODS:MicroRNA microarray and RT-qPCR were used to screen and identify the differential expression of miR-130a between MCF-7/Adr and MCF-7/S.The potential target genes of miR-130a were predicted by online bioinformatic softwares,and PTEN could be a specific target.miR-130a mimics were transfected into MCF-7/S cells,and miR-130a inhibitors were transfected into MCF-7/Adr,respectively.The IC50 of these transfected cells to Adr was evaluated by MTT assay and the expression levels of PTEN mRNA and protein were analyzed by RT-qPCR and Western blot.RESULTS:Compared with the parental MCF-7/S,the relative expression level of miR-130a in MCF-7/Adr was significantly up-regulated to(116 834.70±4 728.32) fold(t=19.035,P=0.000).There were no statistical differences in the level of miR-130a between MCF-7/S and MCF-7/Doc(t=0.703,P=0.521).Compared with control MCF-7/S,the relative expression level of miR-130a in miR-130a mimics-transfected MCF-7/S was significantly increased to(17.686±1.057) folds(t=8.360,P=0.001),and drug resistance of mimics-transfected MCF-7/S cells to Adr became significantly increased.The expression levels of PTEN mRNA and protein in mimics group were down-regulated to(0.362±0.076) folds(t=2.927,P=0.043) and to(0.386±0.020) folds(t=20.713,P=0.000 3).Compared with control MCF-7/Adr,the relative expression level of miR-130a in miR-130a inhibitors-transfected MCF-7/S was significantly decreased to(0.169±0.035) folds(t=12.036,P=0.000 2),and drug resistance of inhibitors-transfected MCF-7/Adr cells to Adr became significantly decreased.The expression levels of PTEN mRNA and protein in inhibitors group were up-regulated to(3.564±0.336) folds(t=4.122,P=0.015) and to(2.019±0.268) folds(t=8.999,P=0.000 8).CONCLUSION:miR-130a may confer breast cancer cell resistance to adriamycin by targeting PTEN.

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OBJECTIVE:To investigate the effect of miR-130a on expression of PTEN,and their association with the chemosensitivity of MCF-7 breast cancer cells to adriamycin.METHODS:MicroRNA microarray and RT-qPCR were used to screen and identify the differential expression of miR-130a between MCF-7/Adr and MCF-7/S.The potential target genes of miR-130a were predicted by online bioinformatic softwares,and PTEN could be a specific target.miR-130a mimics were transfected into MCF-7/S cells,and miR-130a inhibitors were transfected into MCF-7/Adr,respectively.The IC50 of these transfected cells to Adr was evaluated by MTT assay and the expression levels of PTEN mRNA and protein were analyzed by RT-qPCR and Western blot.RESULTS:Compared with the parental MCF-7/S,the relative expression level of miR-130a in MCF-7/Adr was significantly up-regulated to(116 834.70±4 728.32) fold(t=19.035,P=0.000).There were no statistical differences in the level of miR-130a between MCF-7/S and MCF-7/Doc(t=0.703,P=0.521).Compared with control MCF-7/S,the relative expression level of miR-130a in miR-130a mimics-transfected MCF-7/S was significantly increased to(17.686±1.057) folds(t=8.360,P=0.001),and drug resistance of mimics-transfected MCF-7/S cells to Adr became significantly increased.The expression levels of PTEN mRNA and protein in mimics group were down-regulated to(0.362±0.076) folds(t=2.927,P=0.043) and to(0.386±0.020) folds(t=20.713,P=0.000 3).Compared with control MCF-7/Adr,the relative expression level of miR-130a in miR-130a inhibitors-transfected MCF-7/S was significantly decreased to(0.169±0.035) folds(t=12.036,P=0.000 2),and drug resistance of inhibitors-transfected MCF-7/Adr cells to Adr became significantly decreased.The expression levels of PTEN mRNA and protein in inhibitors group were up-regulated to(3.564±0.336) folds(t=4.122,P=0.015) and to(2.019±0.268) folds(t=8.999,P=0.000 8).CONCLUSION:miR-130a may confer breast cancer cell resistance to adriamycin by targeting PTEN.

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Available abstract

OBJECTIVE:To investigate the effect of miR-130a on expression of PTEN,and their association with the chemosensitivity of MCF-7 breast cancer cells to adriamycin.METHODS:MicroRNA microarray and RT-qPCR were used to screen and identify the differential expression of miR-130a between MCF-7/Adr and MCF-7/S.The potential target genes of miR-130a were predicted by online bioinformatic softwares,and PTEN could be a specific target.miR-130a mimics were transfected into MCF-7/S cells,and miR-130a inhibitors were transfected into MCF-7/Adr,respectively.The IC50 of these transfected cells to Adr was evaluated by MTT assay and the expression levels of PTEN mRNA and protein were analyzed by RT-qPCR and Western blot.RESULTS:Compared with the parental MCF-7/S,the relative expression level of miR-130a in MCF-7/Adr was significantly up-regulated to(116 834.70±4 728.32) fold(t=19.035,P=0.000).There were no statistical differences in the level of miR-130a between MCF-7/S and MCF-7/Doc(t=0.703,P=0.521).Compared with control MCF-7/S,the relative expression level of miR-130a in miR-130a mimics-transfected MCF-7/S was significantly increased to(17.686±1.057) folds(t=8.360,P=0.001),and drug resistance of mimics-transfected MCF-7/S cells to Adr became significantly increased.The expression levels of PTEN mRNA and protein in mimics group were down-regulated to(0.362±0.076) folds(t=2.927,P=0.043) and to(0.386±0.020) folds(t=20.713,P=0.000 3).Compared with control MCF-7/Adr,the relative expression level of miR-130a in miR-130a inhibitors-transfected MCF-7/S was significantly decreased to(0.169±0.035) folds(t=12.036,P=0.000 2),and drug resistance of inhibitors-transfected MCF-7/Adr cells to Adr became significantly decreased.The expression levels of PTEN mRNA and protein in inhibitors group were up-regulated to(3.564±0.336) folds(t=4.122,P=0.015) and to(2.019±0.268) folds(t=8.999,P=0.000 8).CONCLUSION:miR-130a may confer breast cancer cell resistance to adriamycin by targeting PTEN.

Key concepts: PTEN, Transfection, MCF-7, MTT assay, Cancer research, microRNA, Molecular biology, Chemistry

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Effects of miR-130a on chemosensitivity of human breast cancer cells to adriamycin by targeting PTEN — Research Paper | ScholarLens