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A METHOD OF PARAFFIN￣EMBEDDING SECTIONS FOLLOWING FLOATING-FROZEN SECLION IMMUNOSTAINING

PR China

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Abstract

Abstract pm frozen sections, after treated by double immunohistochemical staining, are set onthe bottom of embedding box, dehydrated, cut at 1 - 3μm, embedded in paraffin. Then thesections are dried, dehydrated, cleared, and sealed. Under observation the immuno-positivematerial of these sections was located exactly over the cells or fibres. The result is very sat Keyworks: Immunocytochemistry; Doubleimmunostaining; Parafin embedding; Thin section

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What this paper is about

Abstract pm frozen sections, after treated by double immunohistochemical staining, are set onthe bottom of embedding box, dehydrated, cut at 1 - 3μm, embedded in paraffin. Then thesections are dried, dehydrated, cleared, and sealed. Under observation the immuno-positivematerial of these sections was located exactly over the cells or fibres. The result is very sat Keyworks: Immunocytochemistry; Doubleimmunostaining; Parafin embedding; Thin section

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Method / approach

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Available abstract

Abstract pm frozen sections, after treated by double immunohistochemical staining, are set onthe bottom of embedding box, dehydrated, cut at 1 - 3μm, embedded in paraffin. Then thesections are dried, dehydrated, cleared, and sealed. Under observation the immuno-positivematerial of these sections was located exactly over the cells or fibres. The result is very sat Keyworks: Immunocytochemistry; Doubleimmunostaining; Parafin embedding; Thin section

Key concepts: Clearance, Frozen section procedure, Immunostaining, Embedding, Immunocytochemistry, Staining, Immunohistochemistry, Chemistry

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A METHOD OF PARAFFIN￣EMBEDDING SECTIONS FOLLOWING FLOATING-FROZEN SECLION IMMUNOSTAINING — Research Paper | ScholarLens