Changing Trend of Psychrotrophic Methanogens in Different Degraded Wetlands

The experiment is to study psychrophilic methanogens variation during the same period of different degraded wetlands in the Tibetan Plateau. Methanogens were separated through anaerobic separation and rolling tube purification technique in the different samples. Blood cell counting method was used to count methanogens,and their morphological characteristics were observed by fluorescence microscopy,the amount of methane was measured by gas chromatography analysis. The relations between alpine wetland degradation and psychrophilic methanogens were analyzed according to the results. Results showed that with the degradation of wetlands increasing,types and quantities of psychrophilic methanogens are increasing, and the gas production of psychrophilic methanogens also significantly increased. Experiments for psychrotrophic characteristics of methanogens at a level can indicate the extent of degradation of wetlands.