Effects of ShRNA-targeted S100A4 on invasion and migration potential of MCF-7 cells in patients with breast cancer

Objective To study the effects of short hairp in RNA(shRNA) targeting S100A4 on the invasion and migration potential of breast cancer MCF-7 cells.Methods The S100A4-shRNA expression vector was constructed and conformed by sequencing.S100A4-shRNA expression vector was transfected into MCF-7cells via lipofectamine TM 2000,and the changes of S100A4 were determined by using QRT-PCR and Western blot after transfection.S100A4-shRNA expression vector was transfected into breast cancer MCF-7 cells by lipofectamine TM 2000,followed by G418 selection,colon culture.Invasion and migration capability of stably transfected MCF-7 cells in vitro was evaluated by using Transwell chamber model and wound assay.Results S100A4-shRNA expression vector was constructed and transfected into MCF-7cells.48 h after transfection,S100A4 mRNA and S100A4 protein decreased significantly.The morphous of stably transfected MCF-7 cells didn't change notably,but the invasion and migration potentiality in whih decreased significantly.Conclusion S100A4 play an important role in the invasion and metastasis of breast cancer.The possible mechanism may inhibit metastasis of MCF-7 cells by decreasing its invasion and migration capability.