Effects of Neutral Lipids and Tumor Necrosis Factor α on Biological Behavior of Normal Adult Hepatocyte

Objective:To explore the effects of very low density lipoprotein(VLDL),oxidized very low density lipoprotein(OX-VLDL) and tumor necrosis factor α (TNF-α) on proliferation and apoptosis of normal human hepatocyte.Methods:Normal adult hepatocytes were cultivated with VLDL,OX-VLDL and TNF-α of different concentration respectively in serum-free culture medium.Effects of VLDL,OX-VLDL and TNF-α on proliferation of normal adult hepatocyte line were observed by means of MTT measuring respectively.Flow cytometry were used to assess apoptosis rate.Results:1 Cell proliferation of every group was decreased.With doses increased,there was a decreased trend of cell proliferation.Cell proliferation of the VLDL group with concentration of 80μg/ml,the OX-VLDL group with 40μg/ml,80μg/ml and TNF-α group of 1000u/ml and 2000u/ml were significant lower than control group(P0.05).2 TNF-α,combined with OX-VLDL can induce apoptosis. Conclusion:1 Excess of lipids and TNF-α in the liver might damage hepatocyte,and the effects of two factors together on hepatocyte were stronger than one factor alone.2.TNF-α,combined with OX-VLDL can induce apoptosis.