The Study on Expression of TNF-α、IL-10 in Rats with Paraquat Intoxication and the Protection Effect of Rhubarb

Objective: To evaluate the effect of rhubarb on tumor necrosis factor-a(TNF-α) and interleukin-10(IL-10) in rats with paraquat intoxication,and investigate its mechanism.Methods: 90 healthy adult SD rats were randomly divided into Group A(control group n=30),Group B(poisoned group n=30),Group C(rhubarb-treated group n=30).Group B and C were treated intragastrically with PQ at 60 mg/kg,Group C given rhubarb at 300mg/kg.d,while only saline was treated intragastrically the other two groups.Live rats in each group were sacrificed at 6 hours,24 hours and 72 hours,for the determination of TNF-α,IL-10 in plasma of rats.Results: The leves of TNF-α,IL-10 increased at 6 hours after paraquat intoxication in Group B and Group C,and peaked at 72 hours later,with statistical difference from Group A(P0.05,P0.01);Compared with group B,delayed,lower increasing extent,obviously reducing tendency in group C,with statistical difference(P0.01).As to the leves of TNF-α,IL-10and poisoning time,there were high positive correlations among them.Conclusions: Rhubarb ameliorates damage effect caused by PQ poisoning in rats by means of inhibiting the expression of TNF-α,IL-10 in turn to alleviate inflammatory reaction.