Effect of Dietary Selenium on Selenium Deposition and Antioxidant Enzymes Gene Expression in Mouse Kidney

This study was to explore the effect of dietary selenium on selenium deposition and antioxidant enzymes gene expression in mouse kidney.Four groups(Ⅰ,Ⅱ,Ⅲ andⅣ)of mice were fed eight weeks with 0.045,0.1,0.4 and 0.8mg/kg Se,respectively.The results showed that the levels of kidney selenium deposition increased with the increasing of dietary selenium,groupⅠ was significantly lower than other groups(P0.05).Kidney selenium deposition among Ⅱ、Ⅲ、Ⅳ groups showed no significant differences when fed to the 56th day(P0.05).The TrxR2mRNA expression of groupⅠwas significantly lower than that of groupsⅡandⅢ(P0.05),and the two groups also showed significant differences(P0.05).The SOD1and SOD2mRNA expression levels of groupⅠwere significantly lower than that of groupsⅡandⅢ(P0.05).SOD2 expression showed significantly different between groupsⅡ andⅢ(P0.05),while SOD1 expression showed no significant differences between the two groups.The CAT mRNA expression levels of groupsⅠandⅡ were significantly higher than that of groupⅢ(P0.05),there were no significant differences between groupsⅠ and Ⅱ(P0.05).These results indicated that selenium could increase the level of kidney selenium deposition.With the feeding time increased,kidney selenium deposition in high-selenium group showed saturated.Se deficient and excessive could reduce the mRNA expression levels of kidney antioxidant genes(TrxR2,SOD1,SOD2 and CAT).