Effect of Visfatin on Protein Expression of Insulin Signaling Molecules in SW872 Adipocytes

Objective:To evaluate the effects of visfatin on the protein expression of insulin signal molecules including insulin receptor substrate-1(IRS-1),insulin receptor substrate-2(IRS-2) and phosphatidylinositol 3-kinase(PI3K) on the states of insulin resistant in SW872 Adipocytes.Methods:Pre-adipocytes of the line SW872 were cultured and induced to differentiate into matured SW872 adipocytes.Then the cells were treated with oleate at concentration of 1.0mmol/L for 24hto induce insulin resistance.And the cells were cultured with Visfatin at concentration of 100nmol/L for 1h,and then the cell proteins were extracted.Western blot assay was used to detect the protein expression of IRS-1,IRS-2,and PI3K.Results:In the normal states,compared with the control group,100nmol/L visfatin promoted the protein expression of IRS-1,IRS-2,and PI3Kin SW872 adipocytes significantly.The levels of IRS-1,IRS-2,and PI3K were increased respectively by 51.65%(P0.01),44.74%(P0.01),and 61.38%(P0.01).In the insulin resistant states,after the stimulating by visfatin,the protein expression of IRS-1,IRS-2and PI3Kwere increased by 26.98%(P0.05),35.59%(P0.05),and 27.61%(P0.01).Conclusion:Visfatin might promote the glucose transport and play aphysiological role in the insulin resistance in SW872 adipocytes by modulating the signaling molecules of IRS-1,IRS-2,and PI3K.