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2008•Journal of Zhengzhou UniversityRequires access

Influence of berbamine on proliferation and apoptosis of leukemia K562 cells

Qi Shen

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Abstract

Aim: To explore the effects of berbamine(BBM) on proliferation and apoptosis of K562 cells.Methods:K562 cells were allocated into 5 groups,the first 4 groups were treated with 0.8,8.0,40.0,80.0 mg/L BBM,and the control group was given RPMI 1640 for different time.The antiproliferation rate,apoptosis rate,and caspase-3/survivin expression were analyzed by using MTT,flow cytometry,and immunocytochemistry,respectively.Results: The proliferation of K562 cells was significantly inhibited and apoptosis was induced by BBM in time and dose-dependent manner.Immunocytochemistry showed an increase of Caspase-3 and decrease of Survivin expression(P0.05).Conclusion:BBM could inhibit proliferation and induce apoptosis of K562 cells,which may be realized by up-regulation of Caspase-3 expression and down-regulation of Survivin.

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Aim: To explore the effects of berbamine(BBM) on proliferation and apoptosis of K562 cells.Methods:K562 cells were allocated into 5 groups,the first 4 groups were treated with 0.8,8.0,40.0,80.0 mg/L BBM,and the control group was given RPMI 1640 for different time.The antiproliferation rate,apoptosis rate,and caspase-3/survivin expression were analyzed by using MTT,flow cytometry,and immunocytochemistry,respectively.Results: The proliferation of K562 cells was significantly inhibited and apoptosis was induced by BBM in time and dose-dependent manner.Immunocytochemistry showed an increase of Caspase-3 and decrease of Survivin expression(P0.05).Conclusion:BBM could inhibit proliferation and induce apoptosis of K562 cells,which may be realized by up-regulation of Caspase-3 expression and down-regulation of Survivin.

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Available abstract

Aim: To explore the effects of berbamine(BBM) on proliferation and apoptosis of K562 cells.Methods:K562 cells were allocated into 5 groups,the first 4 groups were treated with 0.8,8.0,40.0,80.0 mg/L BBM,and the control group was given RPMI 1640 for different time.The antiproliferation rate,apoptosis rate,and caspase-3/survivin expression were analyzed by using MTT,flow cytometry,and immunocytochemistry,respectively.Results: The proliferation of K562 cells was significantly inhibited and apoptosis was induced by BBM in time and dose-dependent manner.Immunocytochemistry showed an increase of Caspase-3 and decrease of Survivin expression(P0.05).Conclusion:BBM could inhibit proliferation and induce apoptosis of K562 cells,which may be realized by up-regulation of Caspase-3 expression and down-regulation of Survivin.

Key concepts: Survivin, Apoptosis, K562 cells, Immunocytochemistry, Flow cytometry, Cell growth, Chemistry, Molecular biology

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