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2000•Academic Journal of Second Military Medical UniversityRequires access

Matrine inhibits platelet-derived growth factor induced proliferation of mouse skin fibroblasts

Wu Yan

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Abstract

Objective: To test the effect of matrine on platelet derived growth factor BB (PDGF BB) induced proliferation of mouse skin fibroblasts. Methods: Mouse skin fibroblasts were obtained from newborn ICR mice and propagated in vitro . Proliferation of cells was analyzed by mitochondrial reduction of tetrazolium salt MTT to formazan and actual cell count. Results: Matrine (50 250 μg/ml) caused reduction of serum driven cell growth dose dependently. Growth inhibition was totally reversed after removal of the drug. Matrine also produced inhibition of PDGF BB induced cell growth dose dependently. Conclusion: Matrine exhibits potent anti proliferation effect against mouse skin fibroblast. This effect appears to be mediated by a reduction of PDGF BB induced growth. These results suggest that matrine might have therapeutic implication in skin fibrosis. [

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What this paper is about

Objective: To test the effect of matrine on platelet derived growth factor BB (PDGF BB) induced proliferation of mouse skin fibroblasts. Methods: Mouse skin fibroblasts were obtained from newborn ICR mice and propagated in vitro . Proliferation of cells was analyzed by mitochondrial reduction of tetrazolium salt MTT to formazan and actual cell count. Results: Matrine (50 250 μg/ml) caused reduction of serum driven cell growth dose dependently. Growth inhibition was totally reversed after removal of the drug. Matrine also produced inhibition of PDGF BB induced cell growth dose dependently. Conclusion: Matrine exhibits potent anti proliferation effect against mouse skin fibroblast. This effect appears to be mediated by a reduction of PDGF BB induced growth. These results suggest that matrine might have therapeutic implication in skin fibrosis. [

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Available abstract

Objective: To test the effect of matrine on platelet derived growth factor BB (PDGF BB) induced proliferation of mouse skin fibroblasts. Methods: Mouse skin fibroblasts were obtained from newborn ICR mice and propagated in vitro . Proliferation of cells was analyzed by mitochondrial reduction of tetrazolium salt MTT to formazan and actual cell count. Results: Matrine (50 250 μg/ml) caused reduction of serum driven cell growth dose dependently. Growth inhibition was totally reversed after removal of the drug. Matrine also produced inhibition of PDGF BB induced cell growth dose dependently. Conclusion: Matrine exhibits potent anti proliferation effect against mouse skin fibroblast. This effect appears to be mediated by a reduction of PDGF BB induced growth. These results suggest that matrine might have therapeutic implication in skin fibrosis. [

Key concepts: Matrine, Formazan, Platelet-derived growth factor receptor, Platelet-derived growth factor, Fibroblast, Growth factor, Cell growth, Chemistry

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