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Rapid Identification of Lactic Acid Bacteria from Mongolia Home-made Fermented Milk Products Rapidly Identified by 16S rDNA-RFLP Method

Heping Zhang

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Abstract

55 strains of lactic acid bacteria were isolated from traditional homemade fermented milk products in 5 different regions of Mongolia. All strains were identified on basis of restriction fragment length polymorphism (RFLP) analysis and 16S rDNA sequences compared with 17 standard strains. RFLP analysis used a set of restriction enzymes, AluⅠ, HaeⅢ, BsmaⅠ, TspRⅠand HinfⅠ. The results showed that 49 Lactobacilli strains and 2 Pdeiococcus strains were identified to species level, while the other 4 cocci strains were identified at genus level. 16S rDNA-RFLP method was of accuracy, reliability and efficiency for identifying lactic acid bacteria.

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What this paper is about

55 strains of lactic acid bacteria were isolated from traditional homemade fermented milk products in 5 different regions of Mongolia. All strains were identified on basis of restriction fragment length polymorphism (RFLP) analysis and 16S rDNA sequences compared with 17 standard strains. RFLP analysis used a set of restriction enzymes, AluⅠ, HaeⅢ, BsmaⅠ, TspRⅠand HinfⅠ. The results showed that 49 Lactobacilli strains and 2 Pdeiococcus strains were identified to species level, while the other 4 cocci strains were identified at genus level. 16S rDNA-RFLP method was of accuracy, reliability and efficiency for identifying lactic acid bacteria.

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Available abstract

55 strains of lactic acid bacteria were isolated from traditional homemade fermented milk products in 5 different regions of Mongolia. All strains were identified on basis of restriction fragment length polymorphism (RFLP) analysis and 16S rDNA sequences compared with 17 standard strains. RFLP analysis used a set of restriction enzymes, AluⅠ, HaeⅢ, BsmaⅠ, TspRⅠand HinfⅠ. The results showed that 49 Lactobacilli strains and 2 Pdeiococcus strains were identified to species level, while the other 4 cocci strains were identified at genus level. 16S rDNA-RFLP method was of accuracy, reliability and efficiency for identifying lactic acid bacteria.

Key concepts: Restriction fragment length polymorphism, Biology, Bacteria, 16S ribosomal RNA, Lactic acid, Microbiology, Fermentation, Genotype

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Rapid Identification of Lactic Acid Bacteria from Mongolia Home-made Fermented Milk Products Rapidly Identified by 16S rDNA-RFLP Method — Research Paper | ScholarLens