Effect of F10 gene on activities of transcriptional factor NF-κB and AP1

AIM:To investigate the influence of F10 on the activities of transcription factor NF-κB and AP1 in A549 cells.METHODS:The luciferase report plasmids of NF-κB-Luc,AP1-Luc and F10 gene were introduced into A549 cells and the luciferase activity was detected.The DNA binding activities of AP1 and NF-κB in the cells were measured by the electrophoretic mobility shift assay(EMSA).RESULTS:The luciferase activity in F10+ transfection group decreased 30% and increased 2-fold respectively 48 h after transfected with the luciferase report plasmid of NF-κB-Luc and AP1-Luc in A549 cells.The DNA binding activity decreased 49% and increased 23%,respectively.CONCLUSION:F10 gene up-regulates the transcription activity of AP1 and down-regulates the NF-κB in A549 cells.