Specific Cellular and Humoral Immune Responses Induced by DMA Vaccine of HBV Surface Gene in Mice

To observe the specific immune responses and the protection against P815 mastocytoma cells stable expressing HbsAg in H-2d mice after immunized by DNA vaccine of HBV S gene. Methods: The immunization was performed by intramuscular injection of DNA and subcutaneous injection of P815-HBV-S. Serum anti-HBs was detected by ELISA. HbsAg secific cytotoxic T lymphocyte(CTL)activity was measured by 51 Chromium release assay. Results:The expression of HB-sAg was demonstrated by indirect immunofluorescent method. The 450nm A value of serum in immunized mice was 0.87. HBsAg specific CTL activity was 51.1%. HBV-SDNA vaccine could evidently inhibit the tumor growth,prolong the survival period( 38.2 d),and improve the survival rate. Conclusion: pCR3.1-S DNA vaccine has a strong antigenicity and marked killing effect to HBV infected cells in vivo.