Establishment of Local Cerebral Ischemia/Reperfusion Model in Rats and Experience

Objective To explore the method of preparing the model of the focal cerebral ischemia/reperfusion injury in rats.Methods The local cerebral ischemia/reperfusion model in SD rats was established by the modified Longa E.Z.et al.Method.The right middle cerebral artery (MCA) was occluded for 2 hours by intraluminal suture technique and then the reperfusion was performed in 60 SD rats of experimental group.Another 30 rats without occlusion of MCA served as controls.The neurological function was determined at 2 h,24 h,3 d,7 d after the operation in all the rats,and the mortality rate were evaluated at the same time.The infarction extents in model was identified by TTC staining.Results In the experimental group,14% died after the occlusion of MCA.The neurological defect scores were significantly higher at 2 h,24 h,3 d and 7 d after the operation in the experimental group than those in the control group (P0.01).It was revealed by TTC stain that there was obvious ischemic damage in the right frontoparietal lobe,part of temporal lobe and basal ganglia in the experimental group.Conclusion An ideal model of MCAO(Middle Cerebral Artery Occlusion) focal cerebral ischemia/reperfusion could be established in the rats through the improvement of some of the details in the manufacturing process.It has good stability,high reliability,high success rate,and the processe is simple.