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Apoptosis of gastric cancer cells induced by bufalin

Chen Li

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Abstract

In the present paper the mechanism of actions of bufalin on inhibiting human gastric cancer cells was studied MGc-803 cells were treated with bufalin at different concentrations and cell growth was analysed by MTT assay Cell apoptosis was evaluated by light microscopy electric microscopy flow cytometry and DNA argorose gel eletronphoresis It was showed that bufalin at concentration of 0.01 m mol/L inhibited MGc-803 cell growth remarkably the IC 50 was about 0.1 m mol/L Further study showed that bufalin induced MGc-803 cell death characterized by apoptotic phenotypes DNA content changes and chromosome DNA fragmentation Bufalin showed a remarkable cell cycle specificity in inducing apoptosis in G1 phase The induction of apoptosis would be a very important mechanism of bufalin in treatment of gastric cancer

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What this paper is about

In the present paper the mechanism of actions of bufalin on inhibiting human gastric cancer cells was studied MGc-803 cells were treated with bufalin at different concentrations and cell growth was analysed by MTT assay Cell apoptosis was evaluated by light microscopy electric microscopy flow cytometry and DNA argorose gel eletronphoresis It was showed that bufalin at concentration of 0.01 m mol/L inhibited MGc-803 cell growth remarkably the IC 50 was about 0.1 m mol/L Further study showed that bufalin induced MGc-803 cell death characterized by apoptotic phenotypes DNA content changes and chromosome DNA fragmentation Bufalin showed a remarkable cell cycle specificity in inducing apoptosis in G1 phase The induction of apoptosis would be a very important mechanism of bufalin in treatment of gastric cancer

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Available abstract

In the present paper the mechanism of actions of bufalin on inhibiting human gastric cancer cells was studied MGc-803 cells were treated with bufalin at different concentrations and cell growth was analysed by MTT assay Cell apoptosis was evaluated by light microscopy electric microscopy flow cytometry and DNA argorose gel eletronphoresis It was showed that bufalin at concentration of 0.01 m mol/L inhibited MGc-803 cell growth remarkably the IC 50 was about 0.1 m mol/L Further study showed that bufalin induced MGc-803 cell death characterized by apoptotic phenotypes DNA content changes and chromosome DNA fragmentation Bufalin showed a remarkable cell cycle specificity in inducing apoptosis in G1 phase The induction of apoptosis would be a very important mechanism of bufalin in treatment of gastric cancer

Key concepts: Bufalin, Apoptosis, Cell cycle, DNA fragmentation, Flow cytometry, Cancer cell, Fragmentation (computing), Cell

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