The effect of donepezil on expression of CREB in hippocampus of mice with vascular dementia

Objective To study the effect of donepezil on the expression change of cAMP response element binding protein(CREB) in hippocampus of mice with vascular dementia(VD).Methods The models of VD mice were established through three times ischemia-reperfusion of bilateral common carotid arteries.Ninety Kunming mice were randomly divided into sham group(n=30),model group(n=30)and treatment group(treated with donepezil,n=30).The behavioral scores were investigated by step-down test and water maze test at day 29 and 30,respectively.The expression of CREB was examined in hippocampus of mice by immunohistochemistry and Western blot methods.Results The grades of learning and memory of mice treated with donepezil is obviously increased than those in VD mice(P0.05);The expression of CREB in hippocampus of mice treated with donepezil was higher than that in VD mice observed by immunohistochemistry and Western blot methods(P0.05).Conclusion Donepezil could up-regulate the expression of CREB in hippocampus of mice with vascular dementia.