2010•Xinjiang Yike Daxue xuebaoRequires access

Changes in proinflammatory cytokines and its implication during focal cerebral ischemia and reperfusion in rats

Mingyuan Wang

Abstract

Objective To investigate the changes in expression of tumor necrosis factor α(TNF-α) and interleukins 1β(IL-1β) and explore the role of inflammatory cytokines in the focal brain ischemia-reperfusion injury.Methods 20 Sprague-Dawley(SD) rats of either sex(weight 230 to 250 g) were randomly divided into two groups: sham group(n=10) and ischemia/reperfusion(I/R) group(n=10).Using a rat model of cerebral ischemia-reperfusion injury,the changes in expression of TNF-α and IL-1β were observed with immunohistochemistry and content of Evans blue(EB) were examined with enzyme-reader.Results The levels of TNF-α and IL-1β and content of EB were successively inceased(P0.05),in the oschemia-referfusion group compared with those in the sham group.Conclusion The cerebral ischemia/reperfusion injury is correlated with the increasing levels of TNF-α,IL-1β and EB content.The proinflammatory cytokines may participate in the onset and development of cerebral ischemia/reperfusion injury.

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Key concepts: Proinflammatory cytokine, Ischemia, Medicine, Tumor necrosis factor alpha, Reperfusion injury, Immunohistochemistry, Tumor necrosis factor α, Necrosis